Hyphenated Chromatographic Techniques

The hyphenated technique is developed from the coupling of a separation technique and an on-line spectroscopic detection technology. Hyphenated techniques combine chromatographic and spectral methods to exploit the advantages of both. Chromatography produces pure or nearly pure fractions of chemical components in a mixture. Spectroscopy produces selective information for identification using standards or library spectra.  Hyphenated techniques have received ever-increasing attention as the principal means to solve complex analytical problems. Also called double hybrid e.g. –lc-pda-ms; lc-ms-ms; lc-nmr-ms instruments have become available and been applied to pharmaceutical problem solving. It gives faster and accurate analysis, high degree of automation, high sample throughput, separation and quantification achieved at the same time.  Applications include -quantitation of pollutants in drinking and waste water using official U.S. environmental protection agency methods. 2. Quantitation of drug in metabolites and urine is done for the pharmacological and forensic use. 3. Identification of unknown organic compounds in hazardous waste dumps and reaction products by synthetic organic chemistry. 4. Used for drug analysis, pesticide and herbicide detection.

  • GC-MS
  • LC-ITIR
  • LC-MS
  • LC-NMR
  • Chemical fingerprinting and quality control

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