Franz-Josef Meyer-Almes
University of Applied Sciences Darmstadt, Germany
Title: Fluorescence lifetime based assays in drug discovery
Biography
Biography: Franz-Josef Meyer-Almes
Abstract
High-throughput assays for drug screening applications have to fulfill particular specifications. Besides the capability to identify even compounds with low potency, one of the major issues is to minimize the number of false-positive hits in a screening campaign in order to reduce the logistic effort for the subsequent cherry picking and confirmation procedure. In this respect, fluorescence lifetime (FLT) appears as an ideal readout parameter that is supposed to be robust against autofluorescent and lightabsorbing compounds, the most common source of systematic false positives. The extraordinary fluorescence features of the recently drug discovered [1,3]dioxolo[4,5-f][1,3] benzodioxole dyes were exploited to develop FLT-based binding assays for several bacterial and human isoforms of the histone deacetylase (HDAC) family