Yoko Matsumoto
Sojo University, Japan
Title: Therapeutic effects of trehalose liposomes against carcinoma along with apoptosis in vitro and in vivo
Biography
Biography: Yoko Matsumoto
Abstract
Trehalose stabilizes membranes and proteins in cells most likely by hydrogen bonding. Trehalose liposomes (DMTre) composed of DMPC and trehalose micelles have been produced. Hydrodynamic diameter (dhy) of DMTre composed of 30 mol% DMPC and 70 mol% TreC14 was 100 nm with single and narrow range of size distribution, which was preserved for a period remaining stable for more than one month. The thickness of the fixed aqueous layer (TFAL) of DMTreCn was evaluated from the zeta potential and increase in TFAL values of DMTreCn was obtained in a dose-dependent manner. The TFAL values for DMTreCn were larger than that of DMPC liposomes. The remarkable inhibitory effects of DMTre on the growth of human colon, gastric, hepatocellular and lymphoblastic carcinoma cells have been reported. In this study, the inhibitory effects of DMTre on the growth of lung carcinoma (A-549) cells were examined in vitro and in vivo. DMTre inhibited the growth of A-549 cells leading to apoptosis. The activation of caspase-3, 8, and 9 was obtained for A-549 cells treated with DMTre. The suppression of tumor weight of xenograft mice model of carcinoma treated with DMTre was obtained. To investigate induction of apoptosis against tumor, the tissue section of tumor in
xenograft mice model of carcinoma after the treatment with DMTre was dyed and observed using microscope. Many apoptotic brown color cells in the tissue section of tumor was observed, indicating that DMTre could induce apoptosis in tumor cells against xenograft mice model of carcinoma.
