Malkhaz Getia
Tbilisi State Medical University, Georgia
Title: Quantification of some medical preparations of the plant origin
Biography
Biography: Malkhaz Getia
Abstract
Among the biologically active compounds there are various triterpene glycosides (saponins). In recent years some crude extracts from various species of Georgian flora were obtained at the TSMU I Kutateladze Institute of Pharmacochemistry (Tbilisi, Georgia), namely from the leaves of Colchis ivy (Hedera colchica (K. Koch) – extract with antiulcer activity, from the leaves of Fatsia japonica extract with anti-rheumatic activity (Fatsiflogin) and from the leaves of Hedera helix seu H. caucasigena extract with bronchospasmolytic properties (Causuron). Hederacolchiside F (HcF), Fatsiosid D and Hederasaponin C (HsC) were chosen as the biological and chemical markers for the biological active substances. The UV detection is performed at 205 nm. The chromatographic separation was achieved using a reversed phase column C-18. NMR: Structure elucidation of the markers were carried out using 1H NMR (Bruker Avance 400MHz), 13C (Bruker Avance 100 MHz). The proposed HPLC methods are linear in the range studied (r2>0.999) for all the analyses. Precision, sensitivity and linearity are satisfactory in the range studied. Finally, new, simple, sensitive and reproducible HPLC methods have been developed and validated for the simultaneous quantification of HcF, Fatsiosid D and HsC in the crude extract of Colchis ivy, Fatsiflogin and Causuron.
