Hyphenated Chromatographic Techniques for Drug Analysis

The hyphenated technique is developed from the coupling of a separation technique and an on-line spectroscopic detection technology. The remarkable improvements in hyphenated analytical methods over the last two decades have significantly broadened their applications in the analysis of biomaterials, especially natural products. In this article, recent advances in the applications of various hyphenated techniques, e.g., GC-MS, LC-MS, LC-FTIR, LC-NMR, CE-MS, etc. in the context of pre-isolation analyses of crude extracts or fraction from various natural sources, isolation and on-line detection of natural products, chemotaxonomic studies, chemical fingerprinting, quality control of herbal products, dereplication of natural products, and metabolomic studies are discussed with appropriate examples.

  • Chemical studies of separation techniques - HPLC, GC and CE
  • Chemical studies of detection techniques - LC-MS, LC-NMR, LC-IR and LC-PDA
  • Ionization techniques and interfaces
  • Hydrophilic Interaction Chromatography
  • Emerging detection strategies compatible with current separation techniques
  • Biological sample preparation for clinical and forensic applications (RSC)
  • Hyphenated separations for biotechnology and biopharmaceutical analysis (RSC)
  • Green chromatography including environmental science
  • Combination with chemometrics and data-analysis
  • Applications in the chemical and petrochemical industry
  • Hyphenated techniques for natural products, flavours and fragrance analysis
  • Applicability in “Omics” (Metabolomics, Proteomics, Lipidomics)
  • Optical instrumental techniques (UV, infrared, and X-ray spectroscopy)
  • Separation analyses (gas chromatography, HPLC, and thin-layer chromatography)

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