Ruby Kharwar
Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology
Title: Transition metal complexes of New Sulphonamide substituted derivative of 8-Hydroxyquinoline: Synthesis, spectroscopic characterization and in vitro, in silico biological activity & DNA binding studies
Biography
Biography: Ruby Kharwar
Abstract
With vast potential biological activities and metal chelating ability, the utilization of 8-Hydroxyquinoline (8HQ) as a pharmacophore for preparing drugs and coordination compounds has been increased exponentially. The work endeavours synthesis of new sulphonamide substituted derivative of 8HQ which is N-(8-hydroxyquinolin-5-yl)-2,4,6-trimethylbenzenesulfonamide (8HQTMBS) and its metal complexes with metal salts such as Cu(II), Ni(II), Zn(II), Co(II), Fe(II) and Mn(II) have been synthesized. The ligand 8HQTMBS is further characterized by FT-IR, MASS, 1H-NMR, 13C-NMR. Study of physiochemical properties, elemental analysis, FT-IR, and thermal analysis like TGA and DSC were used to confirm the structure of synthesized metal chelates. The ligand and central metals have been coordinated through O and N electron donar sites which are evident from infrared spectra. The antibacterial activity was carried out by agar plate method, the result suggested that the metal chelates increases the efficacy of parent moiety. Also, interaction of Ligand (8HQTMBS) and its metal chelates with calf thymus DNA (CT-DNA) were investigated by UV-Visible spectroscopy, viscosity measurement and gel electrophoresis which showed that the binding of complexes with DNA occurs through intercalation mode. Moreover, molecular docking study was performed using Autodock 4.2 tools to emphasize the experimental behaviour and to investigate antibacterial activities of 8HQTMBS and its metal chelates on proteins of microorganisms such as Bacillus subtilis (5h67), Escherichia coli (3t88), Proteus vulgaris (5i39) and Staphylococcus aureus (3ty7). Further, molecular docking of 8HQTMBS and its metal chelates with double stranded BDNA (PDB ID: 1BNA) confirms the intercalation mode of binding.