Ravindra K Pandey
Roswell Park Cancer Institute
USA
Title: Dual-function near-infrared (nir) photosensitizer for fluorescence imaging and photodynamic therapy (pdt) of cancer
Biography
Biography: Ravindra K Pandey
Abstract
We report herein, the synthesis and biological efficacy of near-infrared (NIR), bacteriochlorin analogs: 3-(1’-butyloxy)ethyl- 3-deacetyl-bacteriopurpurin-18-N-butylimide methyl ester and the corresponding carboxylic acid. In in vitro assays, compared to its methyl ester analog, the corresponding carboxylic acid derivative showed higher photosensitizing efficacy. However, due to drastically different pharmacokinetics in vivo, the desired PS (HPLC purity >99%) showed higher tumor uptake and long-term tumor cure than the corresponding carboxylic acid (HPLC purity > 96.5%) in BALB/c mice bearing colon 26 tumors. Isomerically pure R- and S- isomers of the desired analog (HPLC purity >99%) under similar treatment parameters showed identical efficacy in vitro and in vivo. In addition, the NIR photosensitizer (PS) showed limited skin phototoxicity, and provides an additional advantage over the clinically approved chemically complex hematoporphyrin derivative (Photofrin) as well as other porphyrin-based PDT agents, which makes it a promising dual-function agent for fluorescence-guided surgery with an option of phototherapy of cancer.