Anna Hirsch
Stratingh Institute for Chemistry University of Groningen The Netherlands
Title: Small-molecule modulators of thiamine transport in pathogenic bacteria
Biography
Biography: Anna Hirsch
Abstract
Energy coupling factor (ECF) transporters are a class of ATP-binding cassette (ABC) transporters that mediate the uptake of vitamins in prokaryotes. They consist of an energizing module and a substrate-binding protein (S-component). Different S components can interact with the same energizing module.1.ThiT is the thiamine-specific S-component.2. Based on the cocrystal structure of ThiT-thiamine (1).3 we have designed and synthesized thiamine analogues to identify which residues are the key for substrate binding and to elucidate the mechanism of transport.