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Jonathan S. Foot

Jonathan S. Foot

Pharmaxis Ltd.,
Australia

Title: Development of selective mechanism-based inhibitors for human Semicarbazide-Sensitive Amine Oxidase (SSAO)

Biography

Biography: Jonathan S. Foot

Abstract

Human membrane primary amine oxidase (hAOC3, also known as vascular adhesion protein-1, VAP-1) is a member of the copper dependent amine oxidase family. The enzymatic function of this protein is commonly known as Semicarbazide Sensitive Amine Oxidase (SSAO), and has been shown to play a crucial role in leukocyte rolling, adhesion and migration in various disease models. The binding site of this enzyme contains a topaquinone co-factor, derived from a modified tyrosine residue, that catalyses the oxidative deamination of primary amines to aldehydes with co-committal release ammonia and hydrogen peroxide. As part of our ongoing research into inflammatory lung diseases, we decided to target this enzyme using a mechanism-based inhibitor approach. Here we present the various challenges we have had to address during development including design, selectivity over related amine oxidases, in vitro cell health considerations and most significantly, optimisation of our molecules to be true mechanism-based inhibitors with no substrate turnover. The influence of in vitro SAR profiling and computer aided drug design will be discussed.